Response to “Methodological considerations on the study of diet and fecal levels of Akkermansia muciniphila in older adults”

Authors

  • Fernando Tume Research Group in Metabolism and Molecular Nutrition, Universidad Científica del Sur, Lima, Perú https://orcid.org/0000-0003-2256-4088
  • Edgardo J. Palma-Gutierrez Research Group in Metabolism and Molecular Nutrition, Universidad Científica del Sur, Lima, Perú
  • Víctor Mamani-Urrutia Human Nutrition and Food Research Group (GINAH), Nutrition and Dietetics Program, Universidad Científica del Sur, Lima, Perú
  • Fanny Requena Research Group in Metabolism and Molecular Nutrition, Universidad Científica del Sur, Lima, Perú. Human Nutrition and Food Research Group (GINAH), Nutrition and Dietetics Program, Universidad Científica del Sur, Lima, Perú
  • Joaquim Ruiz Grupo de Investigación en Dinámicas y Epidemiología de la Resistencia a Antimicrobianos-“One Health”, Universidad Científica de Sur, Lima, Perú

DOI:

https://doi.org/10.14306/renhyd.29.4.2848

Keywords:

gut microbiota, elderly, Akkermansia, qPCR

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References

(1) Apaza Durand AJ. Methodological considerations on the study of diet and fecal levels of Akkermansia muciniphila in older adults. Rev Esp Nutr Hum Diet. 2027;31(1),e2728. doi: https://doi.org/10.14306/renhyd.31.1.2827.

(2) Tume F, Palma-Gutierrez EJ, Mamani-Urrutia V, Requena F, Ruiz J. Diet, anthropometric measurements, sleep quality and fecal levels of Akkermansia muciniphila: a cross-sectional study in older adults. Rev Esp Nutr Hum Diet. 2026;30(1):e2596. doi: https://doi.org/10.14306/renhyd.30.1.2596 DOI: https://doi.org/10.14306/renhyd.30.1.2596

(3) Boyer TC, Hanson T, Singer RS. Estimation of Low Quantity Genes: A Hierarchical Model for Analyzing Censored Quantitative Real-Time PCR Data. PLoS ONE. 2013;8(5):e64900. https://doi.org/10.1371/journal.pone DOI: https://doi.org/10.1371/journal.pone.0064900

(4) Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem. 2009;55(4):611–22. https://doi.org/10.1373/clinchem.2008.112797 DOI: https://doi.org/10.1373/clinchem.2008.112797

(5) Tegegne HA, Savidge TC. Gut microbiome metagenomics in clinical practice: bridging the gap between research and precision medicine. Gut Microbes. 17(1):2569739. https://doi.org/10.1080/19490976.2025.2569739 DOI: https://doi.org/10.1080/19490976.2025.2569739

(6) Li X, Lu H. Enhanced metagenomic strategies for elucidating the complexities of gut microbiota: a review. Front Microbiol. 2025;16:1626002. https://doi.org/10.3389/fmicb.2025.1626002 DOI: https://doi.org/10.3389/fmicb.2025.1626002

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Published

2026-06-25

How to Cite

Tume, F., Palma-Gutierrez, E. J., Mamani-Urrutia, V., Requena, F., & Ruiz, J. (2026). Response to “Methodological considerations on the study of diet and fecal levels of Akkermansia muciniphila in older adults”. Spanish Journal of Human Nutrition and Dietetics. https://doi.org/10.14306/renhyd.29.4.2848

Issue

Online First

Section

Letters to the Editor

License

Copyright (c) 2020 Fernando Tume, Edgardo J. Palma-Gutierrez, Víctor Mamani-Urrutia, Fanny Requena, Joaquim Ruiz

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

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